A novel accessory protein ArCel5 from cellulose-gelatinizing fungus Arthrobotrys sp. CX1

Abstract Improved understanding of cellulose swelling mechanism is beneficial for increasing the hydrolysis efficiency cellulosic substrates. Here, we report a family 5 glycoside hydrolase ArCel5 isolated from cellulose-gelatinizing fungus Arthrobotrys sp. CX1. exhibited low specific activity and high capability, which suggested that this protein might function as an accessory protein. Homology modeling glycosylation detection revealed multi-domain including 1 carbohydrate-binding module, linker, catalytic domain. The adsorption capacity, structural changes hydrature index filter paper treated by different mutants demonstrated CBM1 linker played essential role in recognizing, binding decrystallizing substrates, further encouraged synergistic action between cellulases. Notably, modification strengthened region. Overall, our study provides insight into decrystallization novel will benefit future applications. Graphical